pcDNA3-sACE2v1
(Plasmid
#145148)
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PurposeMammalian expression plasmid for soluble ACE2 (protease domain) high affinity variant 1
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 145148 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1(+)
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5332
- Total vector size (bp) 7207
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAngiotensin-converting enzyme 2
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Alt nameACE2
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Alt namepeptidyl-dipeptidase A
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SpeciesH. sapiens (human)
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Insert Size (bp)1875
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MutationExtracellular, soluble protease domain (a.a. M1-D615) with mutations H34A, T92Q, Q325P, A386L
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GenBank IDNM_021804.1
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Entrez GeneACE2 (a.k.a. ACEH)
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CMV-fwd
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2020.03.16.994236v1 for BioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-sACE2v1 was a gift from Erik Procko (Addgene plasmid # 145148 ; http://n2t.net/addgene:145148 ; RRID:Addgene_145148) -
For your References section:
Engineering human ACE2 to optimize binding to the spike protein of SARS coronavirus 2. Chan KK, Dorosky D, Sharma P, Abbasi SA, Dye JM, Kranz DM, Herbert AS, Procko E. Science. 2020 Sep 4;369(6508):1261-1265. doi: 10.1126/science.abc0870. Epub 2020 Aug 4. 10.1126/science.abc0870 PubMed 32753553