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Addgene

pMAK464
(Plasmid #140702)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 140702 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMal-T-Avi-His/BirA
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NBαEGFP

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer caatttcacacaggaaacagccag
  • 3′ sequencing primer gtcctactcaggagagcgttc
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMAK464 was a gift from Takayuki Iwaki (Addgene plasmid # 140702 ; http://n2t.net/addgene:140702 ; RRID:Addgene_140702)

  • For your References section:

    Nanobody production can be simplified by direct secretion from Escherichia coli. Iwaki T, Hara K, Umemura K. Protein Expr Purif. 2020 Feb 13;170:105607. doi: 10.1016/j.pep.2020.105607. 10.1016/j.pep.2020.105607 PubMed 32062022
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