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Addgene

pcDNA3-ABKAR(T/A)
(Plasmid #140052)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 140052 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 5517
  • Total vector size (bp) 7494
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ABKAR(T/A)
  • Species
    S. cerevisiae (budding yeast), Synthetic
  • Insert Size (bp)
    1977
  • Mutation
    Target Thr residue in substrate domain mutated to Ala.
  • Promoter CMV
  • Tags / Fusion Proteins
    • Cerulean3 (N terminal on insert)
    • cpVenus[E172] (C terminal on insert)
    • 6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV forward
  • 3′ sequencing primer BGH reverse
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-ABKAR(T/A) was a gift from Jin Zhang (Addgene plasmid # 140052 ; http://n2t.net/addgene:140052 ; RRID:Addgene_140052)
  • For your References section:

    Polarized activities of AMPK and BRSK in primary hippocampal neurons. Sample V, Ramamurthy S, Gorshkov K, Ronnett GV, Zhang J. Mol Biol Cell. 2015 May 15;26(10):1935-46. doi: 10.1091/mbc.E14-02-0764. Epub 2015 Mar 18. 10.1091/mbc.E14-02-0764 PubMed 25788287