pcDNA3-ABKAR(T/A)
(Plasmid
#140052)
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PurposeNegative control mutant for ABKAR biosensor.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 140052 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3
- Backbone size w/o insert (bp) 5517
- Total vector size (bp) 7494
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameABKAR(T/A)
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SpeciesS. cerevisiae (budding yeast), Synthetic
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Insert Size (bp)1977
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MutationTarget Thr residue in substrate domain mutated to Ala.
- Promoter CMV
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Tags
/ Fusion Proteins
- Cerulean3 (N terminal on insert)
- cpVenus[E172] (C terminal on insert)
- 6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CMV forward
- 3′ sequencing primer BGH reverse (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-ABKAR(T/A) was a gift from Jin Zhang (Addgene plasmid # 140052 ; http://n2t.net/addgene:140052 ; RRID:Addgene_140052) -
For your References section:
Polarized activities of AMPK and BRSK in primary hippocampal neurons. Sample V, Ramamurthy S, Gorshkov K, Ronnett GV, Zhang J. Mol Biol Cell. 2015 May 15;26(10):1935-46. doi: 10.1091/mbc.E14-02-0764. Epub 2015 Mar 18. 10.1091/mbc.E14-02-0764 PubMed 25788287