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Addgene

XLone-Puro eGFP
(Plasmid #140027)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 140027 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    Addgene #96930
  • Backbone size w/o insert (bp) 5721
  • Total vector size (bp) 6438
  • Modifications to backbone
    blasticidin resistant gene switched to puromycin
  • Vector type
    Mammalian Expression, Synthetic Biology
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Enhanced Green Fluorescent Protein
  • Alt name
    eGFP
  • Species
    Synthetic
  • Promoter TRE3G

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KnpI (not destroyed)
  • 3′ cloning site SpeI (not destroyed)
  • 5′ sequencing primer M13 FWD
  • 3′ sequencing primer M13 RVS
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XLone-Puro eGFP was a gift from Xiaoping Bao (Addgene plasmid # 140027 ; http://n2t.net/addgene:140027 ; RRID:Addgene_140027)