pCO489
(Plasmid
#139732)
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PurposeModule plasmid, individual component of Fungal Bioluminescent Pathway, Luz
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 139732 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMOD_D
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Vector typePlant Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepMOD_D_AtuMas:NnLuz:trbcSE9
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SpeciesNeonothopanus nambi
- Promoter AtuMAS
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BsaI (destroyed during cloning)
- 3′ cloning site BsaI (destroyed during cloning)
Resource Information
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A portion of this plasmid was derived from a plasmid made bysynthesized
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCO489 was a gift from Daniel Voytas (Addgene plasmid # 139732 ; http://n2t.net/addgene:139732 ; RRID:Addgene_139732) -
For your References section:
Building customizable auto-luminescent luciferase-based reporters in plants. Khakhar A, Starker CG, Chamness JC, Lee N, Stokke S, Wang C, Swanson R, Rizvi F, Imaizumi T, Voytas DF. Elife. 2020 Mar 25;9. pii: 52786. doi: 10.7554/eLife.52786. 10.7554/eLife.52786 PubMed 32209230