pET-tTALE-EmGFP-15b
(Plasmid
#138280)
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PurposeExpress tTALE-EmGFP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 138280 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET-15b
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5708
- Total vector size (bp) 7457
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Vector typeBacterial Expression, TALEN
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name7-mer tTALE-EmGFP
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Insert Size (bp)1749
- Promoter T7 promoter
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Tag
/ Fusion Protein
- 6x His-tag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer Universal T7 Primer
- 3′ sequencing primer T7 Terminator (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET-tTALE-EmGFP-15b was a gift from Kyubong Jo (Addgene plasmid # 138280 ; http://n2t.net/addgene:138280 ; RRID:Addgene_138280) -
For your References section:
Truncated TALE-FP as DNA Staining Dye in a High-salt Buffer. Shin E, Kim W, Lee S, Bae J, Kim S, Ko W, Seo HS, Lim S, Lee HS, Jo K. Sci Rep. 2019 Nov 20;9(1):17197. doi: 10.1038/s41598-019-53722-0. 10.1038/s41598-019-53722-0 PubMed 31748571