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PurposeAAV genome: expresses the N-terminal of v5 AAV-ABE from the Cbh promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 137177 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepx601
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namev5 AAV-ABE N-terminal
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Alt nameJL645
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gRNA/shRNA sequenceNo U6-sgRNA cassette
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SpeciesSynthetic
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MutationCas9 D10A
- Promoter Cbh
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Inserts were Gibson-cloned into BshTI/BglII-restricted backbones to avoid ITR PCR.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Cbh_v5 AAV-ABE N-terminal was a gift from David Liu (Addgene plasmid # 137177 ; http://n2t.net/addgene:137177 ; RRID:Addgene_137177) -
For your References section:
Cytosine and adenine base editing of the brain, liver, retina, heart and skeletal muscle of mice via adeno-associated viruses. Levy JM, Yeh WH, Pendse N, Davis JR, Hennessey E, Butcher R, Koblan LW, Comander J, Liu Q, Liu DR. Nat Biomed Eng. 2020 Jan;4(1):97-110. doi: 10.1038/s41551-019-0501-5. Epub 2020 Jan 14. 10.1038/s41551-019-0501-5 PubMed 31937940