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Addgene

pBAD-bHs
(Plasmid #134259)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 134259 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    EGFP-pBAD
  • Backbone manufacturer
    Michael Davidson (Addgene #54762)
  • Backbone size w/o insert (bp) 4698
  • Total vector size (bp) 5467
  • Modifications to backbone
    EGFP removed by XhoI-EcoRI digest
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Hyaluronidase from Streptomyces koganeinsis codon-optimized for Salmonella typhimurium
  • Alt name
    bHs
  • Species
    Synthetic; Streptomyces koganeinsis
  • Insert Size (bp)
    759
  • Mutation
    codon optimized for Salmonella typhimurium
  • Promoter AraC
  • Tag / Fusion Protein
    • 6x His (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer TATTTATACTCGAGCATGCCGGTTGCGCGTCGTCTG
  • 3′ sequencing primer TATTTATAGAATTCTCATTACGCCGGCGCGATGGTGGTAAC
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBAD-bHs was a gift from Edwin Manuel (Addgene plasmid # 134259 ; http://n2t.net/addgene:134259 ; RRID:Addgene_134259)
  • For your References section:

    Hyaluronidase-Expressing Salmonella Effectively Targets Tumor-Associated Hyaluronic Acid in Pancreatic Ductal Adenocarcinoma. Ebelt ND, Zuniga E, Passi KB, Sobocinski LJ, Manuel ER. Mol Cancer Ther. 2019 Nov 6. pii: 1535-7163.MCT-19-0556. doi: 10.1158/1535-7163.MCT-19-0556. 10.1158/1535-7163.MCT-19-0556 PubMed 31694889