Skip to main content
Addgene

pGEX-4T-1 EGFP-Sumo2 G
(Plasmid #13383)

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13383 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pGEX-4T-1
  • Backbone manufacturer
    Amersham
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sumo2 G
  • Alt name
    SUMO-2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    300
  • Mutation
    Nonconjugatable SUMO2G: single C-terminal glycine at amino acid position 92
  • GenBank ID
    NP_008868
  • Entrez Gene
    SUMO2 (a.k.a. HSMT3, SMT3B, SMT3H2, SUMO3, Smt3A)
  • Tags / Fusion Proteins
    • GST (N terminal on backbone)
    • EGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer pEGFP-C
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-4T-1 EGFP-Sumo2 G was a gift from Mary Dasso (Addgene plasmid # 13383 ; http://n2t.net/addgene:13383 ; RRID:Addgene_13383)
  • For your References section:

    SUMO-2/3 regulates topoisomerase II in mitosis. Azuma Y, Arnaoutov A, Dasso M. J Cell Biol. 2003 Nov 10. 163(3):477-87. 10.1083/jcb.200304088 PubMed 14597774