pGEX-4T-1 EGFP-Sumo2 G
(Plasmid
#13383)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 13383 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEX-4T-1
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Backbone manufacturerAmersham
- Backbone size w/o insert (bp) 5000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSumo2 G
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Alt nameSUMO-2
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SpeciesH. sapiens (human)
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Insert Size (bp)300
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MutationNonconjugatable SUMO2G: single C-terminal glycine at amino acid position 92
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GenBank IDNP_008868
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Entrez GeneSUMO2 (a.k.a. HSMT3, SMT3B, SMT3H2, SUMO3, Smt3A)
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Tags
/ Fusion Proteins
- GST (N terminal on backbone)
- EGFP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer pEGFP-C (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX-4T-1 EGFP-Sumo2 G was a gift from Mary Dasso (Addgene plasmid # 13383 ; http://n2t.net/addgene:13383 ; RRID:Addgene_13383) -
For your References section:
SUMO-2/3 regulates topoisomerase II in mitosis. Azuma Y, Arnaoutov A, Dasso M. J Cell Biol. 2003 Nov 10. 163(3):477-87. 10.1083/jcb.200304088 PubMed 14597774