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Addgene

Gal tr-lux
(Plasmid #1330)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1330 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pRS316 Gal1-10
  • Backbone size w/o insert (bp) 4895
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Bacterial luciferase-V.h.CTP5
  • Alt name
    Vibrio harveyi luciferase in yeast
  • Species
    Vibrio harveyi CTP5
  • Insert Size (bp)
    2270

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site blunt (destroyed during cloning)
  • 5′ sequencing primer M13R
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    A. Szalay, University of Alberta

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid with selectable Ura marker, maintained i DH5alpha F'.
Using PCRBamH1 and Sac1 sites were created at the ends of luxAB gene (pLx408fab9) and this was cloned into the pRS316 Gal1-10 vector

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Gal tr-lux was a gift from Susan Lindquist (Addgene plasmid # 1330 ; http://n2t.net/addgene:1330 ; RRID:Addgene_1330)
  • For your References section:

    In vivo functions of the Saccharomyces cerevisiae Hsp90 chaperone. Nathan DF, Vos MH, Lindquist S. Proc Natl Acad Sci U S A 1997 Nov 25;94(24):12949-56. 10.1073/pnas.94.24.12949 PubMed 9371781