pET-29b-3xGluGlu-c-jun-His6
(Plasmid
#129227)
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PurposeEncodes 3xGluGlu tagged human c-Jun for purification by nickel affinity
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 129227 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET 29b
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Backbone manufacturerEMD Biosciences
- Backbone size w/o insert (bp) 5370
- Total vector size (bp) 6423
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namecJun
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SpeciesH. sapiens (human)
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Insert Size (bp)1058
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GenBank IDNM_002228
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Entrez GeneJUN (a.k.a. AP-1, AP1, c-Jun, cJUN, p39)
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Tag
/ Fusion Protein
- 6xHistidine (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site SacI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 Term (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Depositor uses BL21 for protein production.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET-29b-3xGluGlu-c-jun-His6 was a gift from Kevin Janes (Addgene plasmid # 129227 ; http://n2t.net/addgene:129227 ; RRID:Addgene_129227) -
For your References section:
An ultrasensitive fiveplex activity assay for cellular kinases. Smolko CM, Janes KA. Sci Rep. 2019 Dec 19;9(1):19409. doi: 10.1038/s41598-019-55998-8. 10.1038/s41598-019-55998-8 PubMed 31857650