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Addgene

pET-28b(+)-DR RecR(34-220)
(Plasmid #12618)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12618 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-28b(+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5368
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Deinococcus radiodurans RecR(34-220)
  • Alt name
    DR RecR(34-220)
  • Species
    Deinococcus radiodurans
  • Insert Size (bp)
    561
  • Mutation
    deleted amino acids 1-33
  • GenBank ID
    AE000513
  • Tags / Fusion Proteins
    • His (N terminal on backbone)
    • His (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-28b(+)-DR RecR(34-220) was a gift from SeWon Suh (Addgene plasmid # 12618 ; http://n2t.net/addgene:12618 ; RRID:Addgene_12618)

  • For your References section:

    Ring-shaped architecture of RecR: implications for its role in homologous recombinational DNA repair. Lee BI, Kim KH, Park SJ, Eom SH, Song HK, Suh SW. EMBO J. 2004 May 19. 23(10):2029-38. 10.1038/sj.emboj.7600222 PubMed 15116069
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