pET-21a(+)-DR RecR
(Plasmid
#12617)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 12617 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET21a(+)
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5443
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameDeinococcus radiodurans RecR
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Alt nameDR RecR
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SpeciesDeinococcus radiodurans
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Insert Size (bp)660
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GenBank IDAE000513
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Entrez GenerecR (a.k.a. DR_0198)
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Tag
/ Fusion Protein
- His (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET-21a(+)-DR RecR was a gift from SeWon Suh (Addgene plasmid # 12617 ; http://n2t.net/addgene:12617 ; RRID:Addgene_12617) -
For your References section:
Ring-shaped architecture of RecR: implications for its role in homologous recombinational DNA repair. Lee BI, Kim KH, Park SJ, Eom SH, Song HK, Suh SW. EMBO J. 2004 May 19. 23(10):2029-38. 10.1038/sj.emboj.7600222 PubMed 15116069