PD-1-miSFIT-6-C, 16-C
(Plasmid
#124681)
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PurposeTuning expression of PD-1
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 124681 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneAB.pCCL.sin.cPPT.GFP.miR-17-3p.sensor.PGK.dNGFR.WPRE
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Backbone manufacturerAddgene: 85866
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePD-1
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SpeciesH. sapiens (human)
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Entrez GenePDCD1 (a.k.a. CD279, PD-1, PD1, SLEB2, hPD-1, hPD-l, hSLE1)
- Promoter minimal CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Bi-directional expression vector driving PD-1 under control of a miR-17 MRE with NGFR as an internal control
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PD-1-miSFIT-6-C, 16-C was a gift from Tudor Fulga (Addgene plasmid # 124681 ; http://n2t.net/addgene:124681 ; RRID:Addgene_124681) -
For your References section:
Precise tuning of gene expression levels in mammalian cells. Michaels YS, Barnkob MB, Barbosa H, Baeumler TA, Thompson MK, Andre V, Colin-York H, Fritzsche M, Gileadi U, Sheppard HM, Knapp DJHF, Milne TA, Cerundolo V, Fulga TA. Nat Commun. 2019 Feb 18;10(1):818. doi: 10.1038/s41467-019-08777-y. 10.1038/s41467-019-08777-y PubMed 30778069