Skip to main content
Addgene

pBabe puro IkB alpha M
(Plasmid #12332)

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12332 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBABE puro
  • Backbone manufacturer
    Available at Addgene (plasmid #1764)
  • Backbone size w/o insert (bp) 5169
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IkB alpha M
  • Alt name
    IkBaM
  • Alt name
    I kappa B alpha mutant
  • Species
    M. musculus (mouse)
  • Mutation
    Dominant-negative mutant. Many signal transduction pathways resulting in NF-B activation culminate in a serine phosphorylation of IkB on residues 32 and 36. Phosphorylation of the COOH-terminal PEST sequence has been implicated in constitutive turnover of IkB. We combined the NH2- and COOH-terminal phosphorylation mutants into a single cDNA for this plasmid.
  • Entrez Gene
    Nfkbia (a.k.a. Nfkbi)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabe puro IkB alpha M was a gift from Inder Verma (Addgene plasmid # 12332 ; http://n2t.net/addgene:12332 ; RRID:Addgene_12332)