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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 12329 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMX
- Backbone size w/o insert (bp) 4500
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameIkB alpha M
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Alt nameIkBaM
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Alt nameI kappa B alpha mutant
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SpeciesM. musculus (mouse)
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MutationDominant-negative mutant. Many signal transduction pathways resulting in NF-B activation culminate in a serine phosphorylation of IkB on residues 32 and 36. Phosphorylation of the COOH-terminal PEST sequence has been implicated in constitutive turnover of IkB. We combined the NH2- and COOH-terminal phosphorylation mutants into a single cDNA for this plasmid.
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Entrez GeneNfkbia (a.k.a. Nfkbi)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
IkB alpha M was generated by digestion of plasmids pCMX-IkBS32/36A and pCMX-IkBMutF with Eco NI and Bst EII and ligation of the small S32/36A fragment to the large vector/IkBMutF fragment.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMX IkB alpha M was a gift from Inder Verma (Addgene plasmid # 12329 ; http://n2t.net/addgene:12329 ; RRID:Addgene_12329) -
For your References section:
Suppression of TNF-alpha-induced apoptosis by NF-kappaB. Van Antwerp DJ, Martin SJ, Kafri T, Green DR, Verma IM. Science. 1996 Nov 1. 274(5288):787-9. 10.1126/science.274.5288.787 PubMed 8864120