pSB118 - pL2_pSB90_fLUC-I_HcPro
(Plasmid
#123193)
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Purposebinary plant vector for transient TEV HcPro and fLUC (with intron) expression
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 123193 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSB90
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Backbone manufacturerModified from pAGM4723 (Weber et al., 2011; DOI:10.1371/journal.pone.0016765) by the addition of the VirGN54D gene
- Backbone size w/o insert (bp) 5851
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Vector typePlant Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name2x35S::HcPro::tNOS 2x35S::fLUC-I::tNOS
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SpeciesSynthetic
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Insert Size (bp)5491
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Transient fLUC and TEV HcPro (silencing suppressor) expression vector for plants. Introns avoid expression of fLUC in Agrobacteria. Increased virulence in Agro. through VirGN54D in the vector backbone
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSB118 - pL2_pSB90_fLUC-I_HcPro was a gift from Erin Cram & Carolyn Lee-Parsons (Addgene plasmid # 123193 ; http://n2t.net/addgene:123193 ; RRID:Addgene_123193) -
For your References section:
EASI Transformation: An Efficient Transient Expression Method for Analyzing Gene Function in Catharanthus roseus Seedlings. Mortensen S, Bernal-Franco D, Cole LF, Sathitloetsakun S, Cram EJ, Lee-Parsons CWT. Front Plant Sci. 2019 Jun 11;10:755. doi: 10.3389/fpls.2019.00755. eCollection 2019. 10.3389/fpls.2019.00755 PubMed 31263474