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pAC10114_pAAVS1-Nst-EF1aBlast2ArtTA3(-)_TetO- NpuDnaE(C)-Hygro(90-341)-P2A-NTR-E2A-mCherry
(Plasmid #122834)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 122834 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pAAVS1-Nst-CAG-DEST (Addgene #80489)
  • Modifications to backbone
    Replaced CAG-DEST with EF1aBlast2ArtTA3(-)_TetO-DEST
  • Vector type
    CRISPR ; Donor vector
  • Selectable markers
    Neomycin (select with G418), Blasticidin ; Dox-inducible NpuDnaE(C)-Hygro(90-341)-P2A-NTR-E2A-mCherry

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NpuDnaE(C)-Hygro(90-341)-P2A-NTR-E2A-mCherry
  • Species
    Synthetic

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer taggcgtgtacggtgggagg
  • 3′ sequencing primer ACCGAGGAGAGGGTTAGGGAT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/452979v2 for BioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAC10114_pAAVS1-Nst-EF1aBlast2ArtTA3(-)_TetO- NpuDnaE(C)-Hygro(90-341)-P2A-NTR-E2A-mCherry was a gift from Albert Cheng (Addgene plasmid # 122834)

  • For your References section:

    Split Selectable Markers. Jillette N, Du M, Cheng AW. bioRxiv 452979 10.1101/452979
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