-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 12205 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepJ3M
- Backbone size w/o insert (bp) 3500
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameMst2
-
SpeciesH. sapiens (human)
-
Insert Size (bp)2000
-
Entrez GeneSTK3 (a.k.a. KRS1, MST2)
-
Tag
/ Fusion Protein
- Myc (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer SV40pro-F (Common Sequencing Primers)
Resource Information
-
Articles Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Addgene's quality control sequence shows a mutation at amino acid E331D of STK3. This mutation is outside the kinase domain and the SARAH dimerization domain, and hence should not affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pJ3M-Mst2 was a gift from Jonathan Chernoff (Addgene plasmid # 12205 ; http://n2t.net/addgene:12205 ; RRID:Addgene_12205) -
For your References section:
The Ste20-like protein kinase, Mst1, dimerizes and contains an inhibitory domain. Creasy CL, Ambrose DM, Chernoff J. J Biol Chem. 1996 Aug 30. 271(35):21049-53. 10.1074/jbc.271.35.21049 PubMed 8702870