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PurposeBarcoded lentiviral vector to express RUNX1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 120475 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneUnknown
- Backbone size w/o insert (bp) 9514
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Vector typeLentiviral
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRUNX1
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SpeciesH. sapiens (human)
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Insert Size (bp)1440
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Entrez GeneRUNX1 (a.k.a. AML1, AML1-EVI-1, AMLCR1, CBF2alpha, CBFA2, EVI-1, PEBP2aB, PEBP2alpha)
- Promoter EF1a
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer 5'-ttctcaagcctcagacagtgg-3' (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
5' cloning site: BamHI (not destroyed), 3' cloning site: BamHI (not destroyed).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EF1a_RUNX1_P2A_Hygro_Barcode was a gift from Prashant Mali (Addgene plasmid # 120475 ; http://n2t.net/addgene:120475 ; RRID:Addgene_120475) -
For your References section:
Mapping Cellular Reprogramming via Pooled Overexpression Screens with Paired Fitness and Single-Cell RNA-Sequencing Readout. Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P. Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 10.1016/j.cels.2018.10.008. 10.1016/j.cels.2018.10.008 PubMed 30448000