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Addgene

pCDM8 hCD81
(Plasmid #11588)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11588 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCDM8
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Tetracycline, 100 & 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Unknown
  • Growth instructions
    The host strain is MC1061/P3. Select for plasmid using 12.5 ug/mL ampicillin and 7.5 ug/mL tetracycline. (supF)
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CD81
  • Alt name
    TAPA-1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1500
  • GenBank ID
    NM_004356
  • Entrez Gene
    CD81 (a.k.a. CVID6, S5.7, TAPA1, TSPAN28)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BstXI (not destroyed)
  • 3′ cloning site BstXI (not destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDM8 hCD81 was a gift from Shoshana Levy (Addgene plasmid # 11588 ; http://n2t.net/addgene:11588 ; RRID:Addgene_11588)
  • For your References section:

    TAPA-1, the target of an antiproliferative antibody, defines a new family of transmembrane proteins. Oren R, Takahashi S, Doss C, Levy R, Levy S. Mol Cell Biol. 1990 Aug . 10(8):4007-15. PubMed 1695320