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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 11588 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCDM8
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 4400
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Tetracycline, 100 & 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Unknown
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Growth instructionsThe host strain is MC1061/P3. Select for plasmid using 12.5 ug/mL ampicillin and 7.5 ug/mL tetracycline. (supF)
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCD81
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Alt nameTAPA-1
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SpeciesH. sapiens (human)
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Insert Size (bp)1500
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GenBank IDNM_004356
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Entrez GeneCD81 (a.k.a. CVID6, S5.7, TAPA1, TSPAN28)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BstXI (not destroyed)
- 3′ cloning site BstXI (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDM8 hCD81 was a gift from Shoshana Levy (Addgene plasmid # 11588 ; http://n2t.net/addgene:11588 ; RRID:Addgene_11588) -
For your References section:
TAPA-1, the target of an antiproliferative antibody, defines a new family of transmembrane proteins. Oren R, Takahashi S, Doss C, Levy R, Levy S. Mol Cell Biol. 1990 Aug . 10(8):4007-15. PubMed 1695320