pCMV-myc-Sema4A RNAiR
(Plasmid
#115172)
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Purposeexpresses RNAi-resistant Sema4A with N-terminal myc tag
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 115172 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV
- Backbone size w/o insert (bp) 4000
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSema4A
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Alt nameSemaphorin 4A
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SpeciesM. musculus (mouse)
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Insert Size (bp)2200
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MutationSilent mutation between amino acids in ORF (401-407) to make resistant to Sema4A shRNA 42-3n.
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GenBank IDNM_013658.3
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Entrez GeneSema4a (a.k.a. AI132332, SemB, Semab)
- Promoter CMV
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Tag
/ Fusion Protein
- myc (N terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer gcttatggccatggaggcccgaACTGGTGGTCAGGGGCCC
- 3′ sequencing primer tcgagagatctcggtcgaccgTCAAGCCACTTCGGCGCC (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-myc-Sema4A RNAiR was a gift from Suzanne Paradis (Addgene plasmid # 115172 ; http://n2t.net/addgene:115172 ; RRID:Addgene_115172) -
For your References section:
Class 4 Semaphorins and Plexin-B receptors regulate GABAergic and glutamatergic synapse development in the mammalian hippocampus. McDermott JE, Goldblatt D, Paradis S. Mol Cell Neurosci. 2018 Jul 4;92:50-66. doi: 10.1016/j.mcn.2018.06.008. 10.1016/j.mcn.2018.06.008 PubMed 29981480