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Addgene

pCMV-myc-Sema4A RNAiR
(Plasmid #115172)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 115172 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCMV
  • Backbone size w/o insert (bp) 4000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Sema4A
  • Alt name
    Semaphorin 4A
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2200
  • Mutation
    Silent mutation between amino acids in ORF (401-407) to make resistant to Sema4A shRNA 42-3n.
  • GenBank ID
    NM_013658.3
  • Entrez Gene
    Sema4a (a.k.a. AI132332, SemB, Semab)
  • Promoter CMV
  • Tag / Fusion Protein
    • myc (N terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer gcttatggccatggaggcccgaACTGGTGGTCAGGGGCCC
  • 3′ sequencing primer tcgagagatctcggtcgaccgTCAAGCCACTTCGGCGCC
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-myc-Sema4A RNAiR was a gift from Suzanne Paradis (Addgene plasmid # 115172 ; http://n2t.net/addgene:115172 ; RRID:Addgene_115172)
  • For your References section:

    Class 4 Semaphorins and Plexin-B receptors regulate GABAergic and glutamatergic synapse development in the mammalian hippocampus. McDermott JE, Goldblatt D, Paradis S. Mol Cell Neurosci. 2018 Jul 4;92:50-66. doi: 10.1016/j.mcn.2018.06.008. 10.1016/j.mcn.2018.06.008 PubMed 29981480