DH10B-M2.NmeMC58II
(Bacterial strain
#114268)
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PurposeThe strain expresses M2.NmeMC58II methylase and zeocin selection marker from the arsB locus.
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Depositing Lab
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Sequence Information
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Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Bacterial Strain | 114268 | Bacteria in agar stab | 1 | $85 |
Backbone
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Vector backboneNone
Growth in Bacteria
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Bacterial Resistance(s)Bleocin (Zeocin), 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B-M2.NmeMC58II
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Growth instructionsThe strain is resistant to 25 µg/ml zeocin selection. Genotype:F– mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara-leu)7697 galU galK λ– rpsL(StrR) nupG arsB::M2.NmeMC58II-ble(ZeoR)
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNone
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
See Supplemental Documents for strain verification protocols and data.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
DH10B-M2.NmeMC58II was a gift from Christopher A. O'Callaghan (Addgene plasmid # 114268) -
For your References section:
MetClo: methylase-assisted hierarchical DNA assembly using a single type IIS restriction enzyme. Lin D, O'Callaghan CA. Nucleic Acids Res. 2018 Jul 9. pii: 5050631. doi: 10.1093/nar/gky596. 10.1093/nar/gky596 PubMed 29986052