pMXBC_bFq
(Plasmid
#114253)
-
PurposePOC12395, MetClo assembly vector with F replication origin and chloramphenicol resistance for BsaI-based MetClo, adaptor sequence type b-q
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 114253 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepMXBC
-
Vector typeSynthetic Biology
Growth in Bacteria
-
Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameLacZalpha
-
SpeciesSynthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pMXBC_bFq was a gift from Christopher A. O'Callaghan (Addgene plasmid # 114253 ; http://n2t.net/addgene:114253 ; RRID:Addgene_114253) -
For your References section:
MetClo: methylase-assisted hierarchical DNA assembly using a single type IIS restriction enzyme. Lin D, O'Callaghan CA. Nucleic Acids Res. 2018 Jul 9. pii: 5050631. doi: 10.1093/nar/gky596. 10.1093/nar/gky596 PubMed 29986052