pGDP1 NDM-1
(Plasmid #112883)

• Purpose: This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.
• Depositing Lab: Gerard Wright
• Publication: Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109. doi: 10.1016/j.chembiol.2016.11.011. Epub 2016 Dec 22.

Backbone

• Vector backbone: pBR322
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: NDM-1
• Species: Klebsiella pneumoniae
• Entrez Gene: blaNDM-1 (a.k.a. D647_p47098)
• Promoter: Pbla
• Tag / Fusion Protein:
  • 6xHis (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: CATGAGACAATAACCCTG
• 3′ sequencing primer: CCAACTCAGCTTCCTTTC

Resource Information

• Supplemental Documents:
  • Minimal ARP Protocol
  • Minimal ARP Map

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that some discrepancies were found between Addgene's quality control and the depositor's insert sequence. The depositor noted that these discrepancies do NOT affect plasmid function. JoVE antibiotic dereplication paper: Zubyk, H.L., Cox, G., Wright, G.D. Antibiotic Dereplication Using the Antibiotic Resistance Platform. J. Vis. Exp. (152), e60536, doi:10.3791/60536 (2019). (https://www.jove.com/video/60536/antibiotic-dereplication-using-the-antibiotic-resistance-platform)