pMM627
(Plasmid
#112532)
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PurposepZE2-PLhrtO-lux-hrtR-RBS2-chuA - Composite plasmid of pMM534 and pMM549, ColE1 origin, KanR
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 112532 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonePZE2
- Backbone size w/o insert (bp) 2216
- Total vector size (bp) 10928
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameHrtR
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SpeciesLactococcus lactis
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Insert Size (bp)570
- Promoter ProD
Cloning Information for Gene/Insert 1
- Cloning method Gibson Cloning
- 5′ sequencing primer GTC AAT ACG AGG ACG CAG AAA GGG TG (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameChuA
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SpeciesE. coli
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Insert Size (bp)1983
- Promoter J23107
Cloning Information for Gene/Insert 2
- Cloning method Gibson Cloning
- 5′ sequencing primer CCG TTC GTA AGC CAT TTC CGC TC (Common Sequencing Primers)
Gene/Insert 3
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Gene/Insert nameluxCDABE
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Alt nameBacterial luciferase operon
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SpeciesPhotorhabdus luminescens
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Insert Size (bp)5798
- Promoter PL(HrtO)
Cloning Information for Gene/Insert 3
- Cloning method Gibson Cloning
- 5′ sequencing primer GGC AAG AAA GCC ATC CAG TTT ACT TTG C (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that Addgene NGS results identified an IS4-like element (ISVsa5 family) immediately downstream of ChuA. The depositing lab indicates that this is not expected to affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMM627 was a gift from Timothy Lu (Addgene plasmid # 112532 ; http://n2t.net/addgene:112532 ; RRID:Addgene_112532) -
For your References section:
An ingestible bacterial-electronic system to monitor gastrointestinal health. Mimee M, Nadeau P, Hayward A, Carim S, Flanagan S, Jerger L, Collins J, McDonnell S, Swartwout R, Citorik RJ, Bulovic V, Langer R, Traverso G, Chandrakasan AP, Lu TK. Science. 2018 May 25;360(6391):915-918. doi: 10.1126/science.aas9315. 10.1126/science.aas9315 PubMed 29798884