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Addgene

pMM627
(Plasmid #112532)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112532 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    PZE2
  • Backbone size w/o insert (bp) 2216
  • Total vector size (bp) 10928
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    HrtR
  • Species
    Lactococcus lactis
  • Insert Size (bp)
    570
  • Promoter ProD

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    ChuA
  • Species
    E. coli
  • Insert Size (bp)
    1983
  • Promoter J23107

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    luxCDABE
  • Alt name
    Bacterial luciferase operon
  • Species
    Photorhabdus luminescens
  • Insert Size (bp)
    5798
  • Promoter PL(HrtO)

Cloning Information for Gene/Insert 3

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that Addgene NGS results identified an IS4-like element (ISVsa5 family) immediately downstream of ChuA. The depositing lab indicates that this is not expected to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMM627 was a gift from Timothy Lu (Addgene plasmid # 112532 ; http://n2t.net/addgene:112532 ; RRID:Addgene_112532)
  • For your References section:

    An ingestible bacterial-electronic system to monitor gastrointestinal health. Mimee M, Nadeau P, Hayward A, Carim S, Flanagan S, Jerger L, Collins J, McDonnell S, Swartwout R, Citorik RJ, Bulovic V, Langer R, Traverso G, Chandrakasan AP, Lu TK. Science. 2018 May 25;360(6391):915-918. doi: 10.1126/science.aas9315. 10.1126/science.aas9315 PubMed 29798884