pMM549
(Plasmid
#112531)
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PurposepZA1D-hrtR-chuA - HrtR expressed consitutively from promoter ProD with RBS2, ChuA expressed consitiutively from promoter J23107, p15a origin, AmpR
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 112531 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepZA1
- Backbone size w/o insert (bp) 2305
- Total vector size (bp) 4989
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameHrtR
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SpeciesLactococcus lactis
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Insert Size (bp)570
- Promoter ProD
Cloning Information for Gene/Insert 1
- Cloning method Gibson Cloning
- 5′ sequencing primer GCG CAC ATT TCC CCG AAA AGT GC (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameChuA
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SpeciesE. coli
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Insert Size (bp)1983
- Promoter J23107
Cloning Information for Gene/Insert 2
- Cloning method Gibson Cloning
- 5′ sequencing primer CCG TTC GTA AGC CAT TTC CGC TC (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMM549 was a gift from Timothy Lu (Addgene plasmid # 112531 ; http://n2t.net/addgene:112531 ; RRID:Addgene_112531) -
For your References section:
An ingestible bacterial-electronic system to monitor gastrointestinal health. Mimee M, Nadeau P, Hayward A, Carim S, Flanagan S, Jerger L, Collins J, McDonnell S, Swartwout R, Citorik RJ, Bulovic V, Langer R, Traverso G, Chandrakasan AP, Lu TK. Science. 2018 May 25;360(6391):915-918. doi: 10.1126/science.aas9315. 10.1126/science.aas9315 PubMed 29798884