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PurposeNMD sensor: pCMV-3XFLAG-Fluc-betaglobin (39PTC)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 112084 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonep3xFLAG-CMV-10
- Backbone size w/o insert (bp) 6204
- Total vector size (bp) 8717
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name3XFLAG firefly luciferase-beta-globin 39PTC
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Alt nameFLuc NMD sensor
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SpeciesH. sapiens (human), M. musculus (mouse); Photinus pyralis
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Insert Size (bp)2513
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Entrez GeneHBB (a.k.a. CD113t-C, ECYT6, beta-globin)
- Promoter CMV
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Tag
/ Fusion Protein
- FLAG (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer hgH-PA-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byb-globin sequence used in this clone was obtained from Professor Lynne Maquat
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKC-4.06 was a gift from James Inglese (Addgene plasmid # 112084 ; http://n2t.net/addgene:112084 ; RRID:Addgene_112084) -
For your References section:
ICE1 promotes the link between splicing and nonsense-mediated mRNA decay. Baird TD, Cheng KC, Chen YC, Buehler E, Martin SE, Inglese J, Hogg JR. Elife. 2018 Mar 12;7. pii: 33178. doi: 10.7554/eLife.33178. 33178 [pii] PubMed 29528287