FGFR2 N549H 3xFlag
(Plasmid
#110106)
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Purposeexpresses FGFR2 with a single amino acid change in mammalian cells
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 110106 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonep3xFlag-CMV-13
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Backbone manufacturerSigma
- Backbone size w/o insert (bp) 6300
- Total vector size (bp) 8763
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namefibroblast growth factor receptor 2
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Alt nameFGFR2
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SpeciesH. sapiens (human)
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Insert Size (bp)2463
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Mutationmutated Asparagine 549 to Histidine (N549H)
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GenBank IDNM_000141.4
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Entrez GeneFGFR2 (a.k.a. BBDS, BEK, BFR-1, CD332, CEK3, CFD1, ECT1, JWS, K-SAM, KGFR, TK14, TK25)
- Promoter CMV
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Tag
/ Fusion Protein
- Flag (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Xba (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer hGH-PA-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FGFR2 N549H 3xFlag was a gift from Martin Fernandez-Zapico (Addgene plasmid # 110106 ; http://n2t.net/addgene:110106 ; RRID:Addgene_110106)