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Addgene

pEM705 Gsi (Cys)
(Plasmid #109373)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 109373 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pEM705
  • Backbone size w/o insert (bp) 6002
  • Total vector size (bp) 7184
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Gsi chimera
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1182
  • Mutation
    Cys at position 391 (Pertussis Toxin Sensitive)
  • GenBank ID
  • Promoter CAG
  • Tag / Fusion Protein
    • None

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer cttctggcgtgtgaccggcggctc
  • 3′ sequencing primer TTGGCAGAGGGAAAAAGATCTCAGTGG
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEM705 Gsi (Cys) was a gift from Robert Lucas (Addgene plasmid # 109373 ; http://n2t.net/addgene:109373 ; RRID:Addgene_109373)
  • For your References section:

    A live cell assay of GPCR coupling allows identification of optogenetic tools for controlling Go and Gi signaling. Ballister ER, Rodgers J, Martial F, Lucas RJ. BMC Biol. 2018 Jan 16;16(1):10. doi: 10.1186/s12915-017-0475-2. 10.1186/s12915-017-0475-2 [pii] PubMed 29338718