LentiV_Cas9_puro
(Plasmid #108100)

• Purpose: Lentiviral expression plasmid of spCas9 with puromycin resistance gene
• Depositing Lab: Christopher Vakoc
• Publication: Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(18)30109-6. doi: 10.1016/j.molcel.2018.02.011.

Backbone

• Vector backbone: LentiV_puro
• Backbone size w/o insert (bp): 7000
• Total vector size (bp): 11203
• Vector type: Mammalian Expression, Lentiviral, CRISPR
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cas9
• Alt name: S. pyogenes CRISPR-Cas9
• Species: Synthetic
• Insert Size (bp): 4100
• GenBank ID:
• Promoter: EFS promoter
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: AACTGGGAAAGTGATGTCGTGTACTG

Resource Information

• Supplemental Documents:
  • LentiV_Cas9_puro (cc60).ape
• Articles Citing this Plasmid:
  • 37 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry