pLibacceptorV2
(Plasmid
#106250)
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Purpose(Empty Backbone) Recombination-mediated cassette exchange with target genomic loci
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 106250 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneSC101
- Backbone size (bp) 5782
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Vector typeBacterial Expression, Cre/Lox, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Growth instructionsGrowth at 42 degrees (and to some extent, 37 degrees) will heat-cure the plasmid.
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Copy numberLow Copy
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLibacceptorV2 was a gift from Sriram Kosuri (Addgene plasmid # 106250 ; http://n2t.net/addgene:106250 ; RRID:Addgene_106250) -
For your References section:
Systematic Dissection of Sequence Elements Controlling sigma70 Promoters Using a Genomically-Encoded Multiplexed Reporter Assay in E. coli. Urtecho G, Tripp AD, Insigne K, Kim H, Kosuri S. Biochemistry. 2018 Feb 1. doi: 10.1021/acs.biochem.7b01069. 10.1021/acs.biochem.7b01069 PubMed 29388765