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Addgene

pLibacceptorV2
(Plasmid #106250)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 106250 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    SC101
  • Backbone size (bp) 5782
  • Vector type
    Bacterial Expression, Cre/Lox, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Growth at 42 degrees (and to some extent, 37 degrees) will heat-cure the plasmid.
  • Copy number
    Low Copy

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLibacceptorV2 was a gift from Sriram Kosuri (Addgene plasmid # 106250 ; http://n2t.net/addgene:106250 ; RRID:Addgene_106250)
  • For your References section:

    Systematic Dissection of Sequence Elements Controlling sigma70 Promoters Using a Genomically-Encoded Multiplexed Reporter Assay in E. coli. Urtecho G, Tripp AD, Insigne K, Kim H, Kosuri S. Biochemistry. 2018 Feb 1. doi: 10.1021/acs.biochem.7b01069. 10.1021/acs.biochem.7b01069 PubMed 29388765