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Addgene

pLenti- V6.3 lox-dsRED-stop-lox-eGFP-blas
(Plasmid #106171)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 106171 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pLenti6.3/TO/V5-DEST
  • Backbone manufacturer
    invitrogen
  • Backbone size w/o insert (bp) 9351
  • Total vector size (bp) 9185
  • Modifications to backbone
    lox-dsRED-STOP-lox-GFP (from plasmid(Plasmid #32702)) cloned in to pLenti 6.3 (through in-fusion cloning, linearized through PstI and SalI digestion, restriction sites maintained)
  • Vector type
    Mammalian Expression, Lentiviral, Cre/Lox
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    dsRED-express2
  • Species
    Synthetic
  • Insert Size (bp)
    678
  • Promoter CMV

Cloning Information for Gene/Insert 1

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer CGCAAATGGGCGGTAGGCGTG
  • 3′ sequencing primer GTACTGGAACTGGGGGGACAG
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    eGFP
  • Species
    Synthetic
  • Insert Size (bp)
    717
  • Promoter CMV

Cloning Information for Gene/Insert 2

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer GGTCCTTCTTGAGTTTGTAAC
  • 3′ sequencing primer CCATCTAATTCAACAAGAATTGGGACAAC
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    insert derived from plasmid (Plasmid #32702) pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE
  • Articles Citing this Plasmid

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti- V6.3 lox-dsRED-stop-lox-eGFP-blas was a gift from Ewa Snaar-Jagalska (Addgene plasmid # 106171 ; http://n2t.net/addgene:106171 ; RRID:Addgene_106171)
  • For your References section:

    Human melanoma brain metastases cell line MUG-Mel1, isolated clones and their detailed characterization. Heitzer E, Groenewoud A, Meditz K, Lohberger B, Liegl-Atzwanger B, Prokesch A, Kashofer K, Behrens D, Haybaeck J, Kolb-Lenz D, Koefeler H, Riedl S, Schaider H, Fischer C, Snaar-Jagalska BE, de'Jong D, Szuhai K, Zweytick D, Rinner B. Sci Rep. 2019 Mar 11;9(1):4096. doi: 10.1038/s41598-019-40570-1. 10.1038/s41598-019-40570-1 PubMed 30858407