pAAV.hSyn.FLEX.SYFP2-POST-T2A-dTomato.FLEX.WPRE.SV40
(Plasmid
#105983)
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PurposeThis AAV plasmid in the presence of Cre recombinase expresses an extracellular SYFP2 fused to the neuroligin-1 cytoplasmic domain for targeting to the synapse with a cell fill dTomato.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 105983 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV-FLEX
- Backbone size w/o insert (bp) 4894
- Total vector size (bp) 7231
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Vector typeMammalian Expression, AAV, Cre/Lox
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Growth instructionsPlasmid is very unstable, screen clones for recombination. Grow at 28oC if possible.
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameFLEX-SYFP2-POST-T2A-dTomato.FLEX
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SpeciesH. sapiens (human), M. musculus (mouse), S. cerevisiae (budding yeast); Thosea asigna virus, Aequoria victoria
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Insert Size (bp)2337
- Promoter human Synapsin 1
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Tags
/ Fusion Proteins
- SYFP2-POST (N terminal on insert)
- T2A-dTomato (C terminal on insert)
- myc (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BsrGI (not destroyed)
- 3′ cloning site KpnI partial (not destroyed)
- 5′ sequencing primer hSynF
- 3′ sequencing primer WPRErev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV.hSyn.FLEX.SYFP2-POST-T2A-dTomato.FLEX.WPRE.SV40 was a gift from Marcel Bruchez (Addgene plasmid # 105983 ; http://n2t.net/addgene:105983 ; RRID:Addgene_105983) -
For your References section:
Fluorescence-based quantitative synapse analysis for cell-type specific connectomics. Kuljis DA, Park E, Telmer CA, Lee J, Ackerman DS, Bruchez MP, Barth AL. eNeuro. 2019 Sep 23. pii: ENEURO.0193-19.2019. doi: 10.1523/ENEURO.0193-19.2019. 10.1523/ENEURO.0193-19.2019 PubMed 31548370