IL16_PDZ_3
(Plasmid
#103929)
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PurposeProtein expression and purification of IL16 PDZ3 domain
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 103929 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEX-6P-1
- Backbone size w/o insert (bp) 4984
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameIL16
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Alt nameIL16 PDZ1
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SpeciesH. sapiens (human)
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Entrez GeneIL16 (a.k.a. LCF, NIL16, PRIL16, prIL-16)
- Promoter tac
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer pGEX Fwd (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
IL16_PDZ_3 was a gift from Sachdev Sidhu (Addgene plasmid # 103929 ; http://n2t.net/addgene:103929 ; RRID:Addgene_103929) -
For your References section:
A specificity map for the PDZ domain family. Tonikian R, Zhang Y, Sazinsky SL, Currell B, Yeh JH, Reva B, Held HA, Appleton BA, Evangelista M, Wu Y, Xin X, Chan AC, Seshagiri S, Lasky LA, Sander C, Boone C, Bader GD, Sidhu SS. PLoS Biol. 2008 Sep 30;6(9):e239. doi: 10.1371/journal.pbio.0060239. 10.1371/journal.pbio.0060239 PubMed 18828675