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Showing: 1 - 20 of 26583 results

  1. pMH-macroHC

    Plasmid
    #82337
    Purpose
    Provides a C-terminal macro domain and 6xHis tag for expression in E. coli
    Depositor
    Michael Hothorn
    Article
    Wild et al Protein Sci. 2016 Oct 24. doi: 10.1002/p
    Insert
    SPX domain of the Vacuolar Transporter Chaperone 4 (VTC4 Budding Yeast)
    Use
    Tags
    6xHis non-cleavable and macro domain of human his…
    Expression
    Bacterial
    Mutation
    Truncated protein, amino acids 1-178
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    82337-plasmid-map-sequence-id-155830

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  2. pVHed07

    Plasmid
    #82350
    Purpose
    Bxb1/TP901 temporal logic gate with mKate2 and sfGFP in Phi80 chromosomal integration plasmid
    Depositor
    Richard Murray
    Article
    Hsiao et al Mol Syst Biol. 2016 May 17;12(5):869. do
    Inserts
    mKate2 RFP
    Superfolder GFP
    Temporal logic gate with TP901 and Bxb1 attachment sites
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    82350-plasmid-map-sequence-id-169046

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  4. pVHed02

    Plasmid
    #82352
    Purpose
    Bxb1/TP901 temporal logic gate with mKate2 and sfGFP on low-copy plasmid
    Depositor
    Richard Murray
    Article
    Hsiao et al Mol Syst Biol. 2016 May 17;12(5):869. do
    Inserts
    mKate2 RFP
    Superfolder GFP
    Temporal logic gate with TP901 and Bxb1 attachment sites
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    82352-plasmid-map-sequence-id-169038

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  11. rFIXX-bp chain A (IRESneo)

    Plasmid
    #82450
    Purpose
    Chain A of the habu snake venom FIX/FX binding protein with a C-terminal polyhistidine-tag. Cloned into pIRESneo3 (Ampicillin) via 5' NheI and 3' BamHI; IRES vector conferring neomycin resistance.
    Depositor
    Robert Peters
    Insert
    rFIXX-bp chain A
    Use
    Tags
    Polyhistidine
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    T7 RNA polymerase promoter
    Availability
    Academic Institutions and Nonprofits only
    82450-plasmid-map-sequence-id-156201

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  12. rFIXX-bp chain B (IRESpuro)

    Plasmid
    #82451
    Purpose
    Chain B of the habu snake venom FIX/FX binding protein with C-terminal biotin-tag. Cloned into pIRESneo3 (Ampicillin) via 5' NheI and 3' BamHI; IRES vector conferring puromycin resistance.
    Depositor
    Robert Peters
    Insert
    rFIXX-bp chain B
    Use
    Tags
    Biotin
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    T7 RNA polymerase promoter
    Availability
    Academic Institutions and Nonprofits only
    82451-plasmid-map-sequence-id-156993

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  16. pGEX-6P-1-GST-CD(Cbx7)

    Plasmid
    #82525
    Purpose
    Expresses GST-CD (Cbx7) fusion proteins in E. coli. Cbx7 chromodomain only; amino acids 1–62.
    Depositor
    Xiaojun Ren
    Insert
    Chromobox Homolog 7 (CBX7 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    CD(Cbx7) ; amino acids 1–62
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    82525-plasmid-map-sequence-id-156818

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  17. pGEX-6P-1-GST-ATL(Cbx7)

    Plasmid
    #82526
    Purpose
    Expresses GST-CD (Cbx7) fusion proteins in E. coli. Cbx7 ATL region only, amino acids 66–81.
    Depositor
    Xiaojun Ren
    Insert
    Chromobox Homolog 7 (CBX7 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    ATL(Cbx7), amino acids 66–81
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    82526-plasmid-map-sequence-id-156820

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  18. pGEX-6P-1-GST-CD-ATL (Cbx7)

    Plasmid
    #82527
    Purpose
    Expresses mutant GST-CD-ATL (Cbx7) fusion proteins in E. coli. Cbx7 CD-ATL region, amino acids 1–84.
    Depositor
    Xiaojun Ren
    Insert
    Chromobox Homolog 7 (CBX7 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    amino acids 1–84
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    82527-plasmid-map-sequence-id-156822

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  19. pGEX-6P-1-GST-CD-ATLm(Cbx7)

    Plasmid
    #82528
    Purpose
    Expresses mutant GST-CD-ATLm (Cbx7) fusion proteins in E. coli. Cbx7 amino acids 1-84 with mutation of RKR70-72, K75, and KR77-78 to AGA70-72, A75, and AG77-78.
    Depositor
    Xiaojun Ren
    Insert
    Chromobox Homolog 7 (CBX7 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    amino acids 1-84 with mutation of RKR70-72, K75, …
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    82528-plasmid-map-sequence-id-156824

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Showing: 1 - 20 of 26583 results