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Addgene

pREPROII-attB
(Plasmid #86557)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 86557 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    Bluescript
  • Backbone size w/o insert (bp) 2770
  • Total vector size (bp) 14670
  • Vector type
    Mammalian Expression, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Oct4
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1056
  • Entrez Gene
    Pou5f1 (a.k.a. NF-A3, Oct-3, Oct-3/4, Oct-4, Oct3, Oct3/4, Oct4, Otf-3, Otf-4, Otf3, Otf3-rs7, Otf3g, Otf4)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    cMyc
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Myc (a.k.a. Myc2, Niard, Nird, bHLHe39)

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    Sox2
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Sox2 (a.k.a. Sox-2, lcc, ysb)

Cloning Information for Gene/Insert 3

Gene/Insert 4

  • Gene/Insert name
    Kruppel-like factor 4
  • Alt name
    Kruppel-like factor 4
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Klf4 (a.k.a. EZF, Gklf, Zie)

Cloning Information for Gene/Insert 4

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pREPROII-attB was a gift from Ralf Kuehn (Addgene plasmid # 86557 ; http://n2t.net/addgene:86557 ; RRID:Addgene_86557)
  • For your References section:

    Efficient generation of rat induced pluripotent stem cells using a non-viral inducible vector. Merkl C, Saalfrank A, Riesen N, Kuhn R, Pertek A, Eser S, Hardt MS, Kind A, Saur D, Wurst W, Iglesias A, Schnieke A. PLoS One. 2013;8(1):e55170. doi: 10.1371/journal.pone.0055170. Epub 2013 Jan 31. 10.1371/journal.pone.0055170 PubMed 23383095