Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Showing: 21 - 40 of 10306 results

  3. pCRISPRyl

    Plasmid
    #70007
    Purpose
    CRISPR/Cas9 vector for Yarrowia lipolytica, with AvrII site for sgRNA insertion
    Depositor
    Ian Wheeldon
    Article
    Schwartz et al ACS Synth Biol. 2015 Dec 29.
    Inserts
    Codon optimized Cas9 from S. pyogenes
    sgRNA expression cassette
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    SCR1'-tRNA and UAS1B8-TEF(136)
    Availability
    Academic Institutions and Nonprofits only
    70007-plasmid-map-sequence-id-137578

    Enlarge

  6. pENV8(GAAA)-GFPuv

    Plasmid
    #70128
    Purpose
    Negative control cobalamin riboswitch reporter vector for use in E. coli; deletion of regulatory kissing loop interaction by conversion of L5 to GAAA tetra loop
    Depositor
    Robert Batey
    Article
    Johnson JE et al Nature. 2012 Dec 6;492(7427):133-7. doi:
    Insert
    kissing loop regulatory mutant of environmental cobalamin riboswitch sequence (env8)
    Use
    Synthetic Biology
    Tags
    None
    Expression
    Bacterial
    Mutation
    Promoter
    tac
    Availability
    Academic Institutions and Nonprofits only
    70128-plasmid-map-sequence-id-130022

    Enlarge

  17. pJBL-RNAINS3

    Plasmid
    #69455
    Purpose
    RNA-IN S3 variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)
    Depositor
    Julius Lucks
    Article
    Watters et al Nucleic Acids Res. 2015 Sep 8. pii: gkv8
    Insert
    RNA-IN S3
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    mutations for S3 variant (from wt)
    Promoter
    J23119 and J23119 (sigma 70)
    Availability
    Academic Institutions and Nonprofits only
    69455-plasmid-map-sequence-id-122579

    Enlarge

  18. pJBL-RNAINS4

    Plasmid
    #69456
    Purpose
    RNA-IN S4 variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)
    Depositor
    Julius Lucks
    Article
    Watters et al Nucleic Acids Res. 2015 Sep 8. pii: gkv8
    Insert
    RNA-IN S4 + SFGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    mutations for S4 variant (from wt)
    Promoter
    J23119 and J23119 (sigma 70)
    Availability
    Academic Institutions and Nonprofits only
    69456-plasmid-map-sequence-id-122578

    Enlarge

Showing: 21 - 40 of 10306 results