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Addgene
Showing: 1 - 20 of 1730 results

  1. pcDNA3.1 V5 His-TOPO-GluCl optalpha-mYFP L9'F (Opt α-GluClv2.0)

    Plasmid
    #47387
    Purpose
    allow pharmacologically induced silencing of electrical activity in CNS neurons upon exposure to the anthelmintic drug ivermectin
    Depositor
    Henry Lester
    Article
    Frazier et al J Biol Chem. 2013 May 29.
    Insert
    glutamate-gated chloride Channels
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Leu9 prime mutated to F (Phenylalanine in M3-M4 l…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    47387-plasmid-map-sequence-id-67454

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  3. SP6-hCas9-Ce-mRNA

    Plasmid
    #47911
    Purpose
    In vitro transcription of a mRNA encoding humanized Cas9 nuclease, with 3' UTRs suitable for germline expression in C. elegans, for doing CRISPR-Cas by RNA injection. Uses SP6 RNA polymerase.
    Depositor
    Paul Sternberg
    Article
    Chiu et al Genetics. 2013 Aug 26.
    Insert
    hCas9 with C. elegans 5' and 3' UTRs
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    SP6
    Availability
    Academic Institutions and Nonprofits only
    47911-plasmid-map-sequence-id-68528

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  5. 5' EGFP SNF-5 pXOON

    Plasmid
    #48700
    Purpose
    Encodes 5' EGFP - SNF-5 fusion construct for expression in Xenopus oocytes or Mammalian Cells
    Depositor
    Dmitri Boudko
    Insert
    snf-5 (snf-5 Nematode)
    Use
    Xenopus oocyte expression
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    48700-plasmid-map-sequence-id-70541

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  6. CeCollectrin-like pXOOM

    Plasmid
    #48704
    Purpose
    Encodes the Caenorhabditis elegans Collectrin homologue for expressing in Xenopus Oocytes of Mammalian cells
    Depositor
    Dmitri Boudko
    Insert
    NM_058858 (C50F2.7 Nematode)
    Use
    Xenopus oocyte expression
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    t7
    Availability
    Academic Institutions and Nonprofits only

  15. lis-1::L4440

    Plasmid
    #11336
    Depositor
    Guy Caldwell
    Insert
    lis-1 (lis-1 Nematode)
    Use
    RNAi
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  16. nud-1::L4440

    Plasmid
    #11337
    Depositor
    Guy Caldwell
    Insert
    nud-1 (nud-1 Nematode)
    Use
    RNAi
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  17. pLexA::lis-1

    Plasmid
    #11338
    Depositor
    Guy Caldwell
    Insert
    lis-1 (lis-1 Nematode)
    Use
    Tags
    LexA DNA Binding Domain
    Expression
    Yeast
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  18. pLexA::nud-1

    Plasmid
    #11339
    Depositor
    Guy Caldwell
    Insert
    nud-1 (nud-1 Nematode)
    Use
    Tags
    LexA DNA Binding Domain
    Expression
    Yeast
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
Showing: 1 - 20 of 1730 results