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52,149 results

Showing: 101 - 120 of 52149 results

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pINTO-NFH::hSNRNP70

Plasmid

#65926

Purpose

Expression of human SNRNP70 fused to Flag-HA tag (N-terminus)

Depositor

Roberto Bonasio

Article

Beck et al J Proteome Res. 2014 Dec 5;13(12):6135-4

Insert

small nuclear ribonucleoprotein 70kDa (U1) (SNRNP70 Human)

Use

Tags

FLAG-HA

Expression

Mammalian

Mutation

Two silent mutations

Promoter

CMV/TO and CMV/TetO2 (T-REx)

Availability

Academic Institutions and Nonprofits only

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GIV-WT - FLAG

Plasmid

#65947

Purpose

Expression GIV/Girdin in Mammalian cell

Depositor

Pradipta Ghosh

Article

Lin et al Mol Biol Cell. 2014 Nov 5;25(22):3654-71

Insert

GIV/Girdin (CCDC88A Human)

Use

Tags

3xFlag

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
GIV-F1685A FLAG

Plasmid

#65948

Purpose

Expression GIV/Girdin F1685A mutation in Mammalian cell

Depositor

Pradipta Ghosh

Article

Lin et al Mol Biol Cell. 2014 Nov 5;25(22):3654-71

Insert

GIV/Girdin (CCDC88A Human)

Use

Tags

3xFlag

Expression

Mammalian

Mutation

Phenylalanine 1685 to Alanine

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
psicheck_NDUFA6_3UTR_mut

Plasmid

#65971

Purpose

Luciferase assay vector, assessing regulation of NDUFA6 3' UTR, with two mutated tandem CAC motifs

Depositor

Thomas Tuschl

Insert

NDUFA6 (NDUFA6 Human)

Use

Tags

Expression

Mammalian

Mutation

mutated tandem CAC motif to CCC motif

Promoter

Availability

Academic Institutions and Nonprofits only
213-petDuet1-GBD-LUMP--cdc42

Plasmid

#65973

Purpose

Dual expression of GBD-LUMP and cdc42

Depositor

Gerard Marriott

Article

Hoepker et al Proc Natl Acad Sci U S A. 2015 Apr 30. p

Inserts

LUMP
cell division cycle 42 (CDC42 Human)

Use

Tags

GBD (GTPase binding domain from ACK1; residues 44…

Expression

Bacterial

Mutation

constitutively active Cdc42 (Q61L)

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pETDuet-1∆HisTag_hNMT_Nef

Plasmid

#66078

Purpose

Single vector system expressing hNMT1 (81-496 amino acids) and NEF allowing myristoylation of recombinant protein produce in Ecoli system

Depositor

Dieter Willbold

Article

Gluck et al PLoS One. 2010 Apr 9;5(4):e10081. doi: 1

Inserts

NMT (NMT1 Human)
Nef

Use

Tags

Expression

Bacterial

Mutation

Deletion of first 80 amino acids

Promoter

T7-lacO

Availability

Academic Institutions and Nonprofits only

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pGL4.10-VEGFprom(-1000 to -1)

Plasmid

#66128

Purpose

Luciferase-based reporter for VEGF promoter region (-1000 to -1 bp)

Depositor

David Mu

Article

Wood et al Sci Rep. 2016 Feb 25;6:19857. doi: 10.10

Insert

Vascular Endothelial Growth Factor -A promoter region (VEGFA Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

VEGF -1000 to -1

Availability

Academic Institutions and Nonprofits only

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pGL4.10-VEGFprom(-1000 to -500)

Plasmid

#66129

Purpose

Luciferase-based reporter for VEGF promoter region (-1000 to -500 bp)

Depositor

David Mu

Article

Wood et al Sci Rep. 2016 Feb 25;6:19857. doi: 10.10

Insert

Vascular Endothelial Growth Factor -A promoter region (VEGFA Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

VEGF -500 to -1

Availability

Academic Institutions and Nonprofits only

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pGL4.10-VEGFprom(-500 to -1)

Plasmid

#66130

Purpose

Luciferase-based reporter for VEGF promoter region (-500 to -1 bp)

Depositor

David Mu

Article

Wood et al Sci Rep. 2016 Feb 25;6:19857. doi: 10.10

Insert

Vascular Endothelial Growth Factor-A promoter region (VEGFA Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

VEGF -500 to -1

Availability

Academic Institutions and Nonprofits only

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pGL4.10-VEGFprom(-950 to -700)

Plasmid

#66133

Purpose

Luciferase-based reporter for VEGF promoter region (-950 to -700 bp)

Depositor

David Mu

Article

Wood et al Sci Rep. 2016 Feb 25;6:19857. doi: 10.10

Insert

Vascular Endothelial Growth Factor-A promoter region (VEGFA Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

VEGF -950 to -700

Availability

Academic Institutions and Nonprofits only

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Vimentin - APEX2 in pECFP

Plasmid

#66170

Purpose

APEX2 fused to the C-terminus human vimentin

Depositor

Alice Ting

Article

Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

Insert

human Vimentin - APEX2 (VIM Synthetic, Human)

Use

Tags

Flag epitope tag (DYKDDDDK)

Expression

Mammalian

Mutation

APEX2 contains 4 mutations relative to wt soybean…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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APEX2 - Actin in pEGFP

Plasmid

#66172

Purpose

APEX2 fused to the N-terminus of human beta actin

Depositor

Alice Ting

Article

Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

Insert

APEX2 - human beta Actin (ACTB Synthetic, Human)

Use

Tags

Flag epitope tag (DYKDDDDK)

Expression

Mammalian

Mutation

APEX2 contains 4 mutations relative to wt soybean…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pOPINK-TRABID (NZF1-3, aa 1-178)

Plasmid

#66185

Purpose

Expresses TRABID NZF1-3 (aa 1-178) in E. coli

Depositor

David Komander

Article

Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

Insert

TRABID (ZRANB1 Human)

Use

Tags

His6-GST

Expression

Bacterial

Mutation

only codes for amino acids 1-178 of TRABID

Promoter

T7

Availability

Academic Institutions and Nonprofits only
pEGFP-FLAG-AKAP-Lbc

Plasmid

#67571

Purpose

Expresses GFP and FLAG-tagged AKAP-Lbc in mammalian cells

Depositor

Graeme Carnegie

Article

Burmeister et al J Biol Chem. 2015 May 8;290(19):12058-67

Insert

A-kinase anchoring protein Lbc (AKAP13 Human)

Use

Tags

FLAG and GFP

Expression

Mammalian

Mutation

R2604G in AKAP

Promoter

Availability

Academic Institutions and Nonprofits only
FLAG-Cerulean-UCR1

Plasmid

#67578

Purpose

Expresses the UCR1 region of PDE4D3 in mammalian cells

Depositor

Graeme Carnegie

Article

Wang et al Cell Signal. 2015 May;27(5):908-22. doi:

Insert

Upstream conserved region 1 of phosphodiesterase 4D3 (PDE4D Human)

Use

Tags

Cerulean and FLAG

Expression

Mammalian

Mutation

Amino acid residues 17-136 of PDE4D3

Promoter

Availability

Academic Institutions and Nonprofits only
FLAG-RFP-UCR1C

Plasmid

#67582

Purpose

Expresses C-terminal amino acids of UCR1 region of PDE4D3 in mammalian cells

Depositor

Graeme Carnegie

Article

Wang et al Cell Signal. 2015 May;27(5):908-22. doi:

Insert

C-terminus of upstream conserved region 1 of phosphodiesterase 4D3 (PDE4D Human)

Use

Tags

FLAG and TagRFP

Expression

Mammalian

Mutation

Amino acid residues 80-136 of PDE4D3

Promoter

Availability

Academic Institutions and Nonprofits only
GST-PRMT9 WT

Plasmid

#67607

Purpose

bacterial expression of wild type human PRMT9

Depositor

Steven Clarke

Article

Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

Insert

PRMT9 (PRMT9 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

wild type

Promoter

tac

Availability

Academic Institutions and Nonprofits only

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GST-PRMT9 L182A/D183A/I184A/G185A

Plasmid

#67608

Purpose

bacterial expression of catalytically inactive GST-PRMT9 LDIG(182-185)AAAA mutant

Depositor

Steven Clarke

Article

Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

Insert

PRMT9 (PRMT9 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

catalytic mutant LDIG(182-185)AAAA

Promoter

tac

Availability

Academic Institutions and Nonprofits only
GST-PRMT9 D258G mutant

Plasmid

#67609

Purpose

bacterial expression of GST-PRMT9 D258G Double E loop mutant

Depositor

Steven Clarke

Article

Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

Insert

PRMT9 (PRMT9 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

D258G double E loop mutant

Promoter

tac

Availability

Academic Institutions and Nonprofits only
GST-PRMT9 G260E mutant

Plasmid

#67610

Purpose

bacterial expression of GST-PRMT9 G260E Double E loop mutant

Depositor

Steven Clarke

Article

Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

Insert

PRMT9 (PRMT9 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

G260E double E loop mutant

Promoter

tac

Availability

Academic Institutions and Nonprofits only