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8,096 results

Showing: 41 - 60 of 8096 results

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pHL-EF1a-SphcCas9-iP-A

Plasmid

#60599

Purpose

Expresses human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.

Depositor

Akitsu Hotta

Article

Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

Insert

CRISPR Cas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Codon usage optimized for human usage

Promoter

Availability

Academic Institutions and Nonprofits only

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pMXs-puro-RFP-ATG9A

Plasmid

#60609

Purpose

Expresses RFP-hATG9A in mammalian cells

Depositor

Noboru Mizushima

Article

Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

Insert

Homo sapiens autophagy related 9A (PPARG Human)

Use

Retroviral

Tags

RFP

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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YFP NLS Beta-Actin

Plasmid

#60613

Purpose

Encodes YFP and NLS tagged beta-actin

Depositor

Primal de Lanerolle

Article

Chang et al Proc Natl Acad Sci U S A. 2011 May 24;10

Insert

Beta-actin (ACTB Human)

Use

Tags

EYFP and NLS

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pCRCT

Plasmid

#60621

Purpose

Plasmid encoding iCas9, tracrRNA and crRNAs

Depositor

Huimin Zhao

Article

Bao et al ACS Synth Biol. 2014 Sep 19.

Inserts

iCas9
tracrRNA

Use

CRISPR

Tags

FLAG and SV40 NLS

Expression

Yeast

Mutation

changed Aspartate 147 to Tyrosine, Proline 411 to…

Promoter

RPR1p and TEF1p

Availability

Academic Institutions and Nonprofits only

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LV-TRE-WT human MyoD-T2A-dsRedExpress2

Plasmid

#60628

Purpose

Expresses WT human MyoD and dsRedExpress2 in response to doxycycline

Depositor

Charles Gersbach

Article

Kabadi et al ACS Synth Biol. 2014 Dec 10.

Insert

VP64 mouse MyoD (MYOD1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

TRE

Availability

Academic Institutions and Nonprofits only

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LV-TRE-VP64 human MyoD-T2A-dsRedExpress2

Plasmid

#60629

Purpose

Expresses VP64 human MyoD and dsRedExpress2 in response to doxycycline

Depositor

Charles Gersbach

Article

Kabadi et al ACS Synth Biol. 2014 Dec 10.

Insert

VP64 human MyoD (MYOD1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

TRE

Availability

Academic Institutions and Nonprofits only

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pCAG-Kir2.1Mut-T2A-tdTomato

Plasmid

#60644

Purpose

Expresses Kir2.1Mut-T2A-tdTomato

Depositor

Massimo Scanziani

Article

Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

Insert

Kcnj2 (Kcnj2 Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

GYG to AAA (aa144-146)

Promoter

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) CircRNA Mini Vector

Plasmid

#60648

Purpose

Expression plasmid for expressing circular RNAs of a desired sequence

Depositor

Jeremy Wilusz

Article

Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

Insert

ZKSCAN1 (ZKSCAN1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pCAG-mNaChBac-T2A-tdTomato

Plasmid

#60650

Purpose

Expresses mNaChBac-T2A-tdTomato

Depositor

Massimo Scanziani

Article

Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

Insert

mNaChBac-T2A-tdTomato

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-F-FLEX-mNaChBac-T2A-tdTomato

Plasmid

#60658

Purpose

Flp-dependent expression of mNaChBac-T2A-tdTomato

Depositor

Massimo Scanziani

Article

Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

Insert

FLEX-mNaChBac-T2A-tdTomato

Use

AAV

Tags

Expression

Mutation

Promoter

EF1α

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-F-FLEX- Kir2.1-T2A-tdTomato

Plasmid

#60661

Purpose

Flp-dependent expression of Kir2.1-T2A-tdTomato

Depositor

Massimo Scanziani

Article

Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

Insert

FLEX- Kir2.1-T2A-tdTomato

Use

AAV

Tags

Expression

Mutation

Promoter

EF1α

Availability

Academic Institutions and Nonprofits only

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pCAG-Flpo

Plasmid

#60662

Purpose

Expresses pCAG-Flpo

Depositor

Massimo Scanziani

Article

Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

Insert

Flpo

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pAAV-hSynapsin-Flpo

Plasmid

#60663

Purpose

Expresses pAAV-hSynapsin-Flpo

Depositor

Massimo Scanziani

Article

Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

Insert

Flpo

Use

AAV

Tags

Expression

Mutation

Promoter

hSynapsin1

Availability

Academic Institutions and Nonprofits only

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pTara:500

Plasmid

#60717

Purpose

Expresses T7 RNAP from the arabinose inducible promoter PBAD with a RBS strength of 500 from the Salis RBS calculator. Also includes a constitutive araC ORF

Depositor

Matthew Bennett

Article

Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

Insert

T7 RNAP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PBAD

Availability

Academic Institutions and Nonprofits only

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pGL3-Basic-8x-gRNA-eGFP

Plasmid

#60718

Purpose

eGFP reporter containing 8 copies of a gRNA binding site for light-inducible dCas9 activation

Depositor

Charles Gersbach

Article

Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

Insert

8 copies of gRNA binding site (5'-AAAGGTCGAGAAACTGCAAA-3')

Use

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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gRNA-eGFP-Reporter

Plasmid

#60719

Purpose

Expresses guide RNA used to activate pGL3-Basic-8x-gRNA-eGFP reporter.

Depositor

Charles Gersbach

Article

Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

Insert

gRNA (5'-AAAGGTCGAGAAACTGCAAA-3')

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 and mouse U6

Availability

Academic Institutions and Nonprofits only

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pET28:GFP

Plasmid

#60733

Purpose

pET28 driving expression of GFP, biobrick part Bba_E0040

Depositor

Matthew Bennett

Article

Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

Insert

GFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-CaMPARI V398D

Plasmid

#60786

Purpose

Expresses CaMPARI V398D in mammalian cells, a photoconvertible fluorescent protein-based calcium integrator

Depositor

Eric Schreiter, Loren Looger

Article

Fosque et al Science. 2015 Feb 13;347(6223):755-60. d

Insert

CaMPARI V398D

Use

Tags

Nuclear Export Signal

Expression

Mammalian

Mutation

V398D

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CaMPARI W391F V398L

Plasmid

#60787

Purpose

Expresses CaMPARI W391F V398L in mammalian cells, a photoconvertible fluorescent protein-based calcium integrator

Depositor

Eric Schreiter, Loren Looger

Article

Fosque et al Science. 2015 Feb 13;347(6223):755-60. d

Insert

CaMPARI W391F V398L

Use

Tags

Nuclear Export Signal

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-eGFP-53BP1

Plasmid

#60813

Purpose

mammalian expression vector

Depositor

Daniel Durocher

Article

Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

Insert

53BP1 (TP53BP1 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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