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Showing: 61 - 80 of 6966 results

  8. pRS416-PHO5-GFP-Opi1 + terminator

    Plasmid
    #58836
    Purpose
    Expresses GFP-Tagged Opi1 + Terminator in yeast
    Depositor
    Tim Levine
    Insert
    Opi1p (OPI1 Budding Yeast)
    Use
    Tags
    GFP
    Expression
    Yeast
    Mutation
    Promoter
    PHO5
    Availability
    Academic Institutions and Nonprofits only

  9. pRS414-7x2-PHO5-GFP-hPLC delta PH domain dimer

    Plasmid
    #58837
    Purpose
    Expresses GFP-Tagged Human PLC delta PH domain in yeast
    Depositor
    Tim Levine
    Insert
    2x Phospolipase C delta1 PH domain (Plcd1 Rat)
    Use
    Tags
    GFP
    Expression
    Yeast
    Mutation
    Promoter
    PHO5
    Availability
    Academic Institutions and Nonprofits only

  12. pEVA1

    Plasmid
    #58953
    Purpose
    Contains a synthetic DNA sequence, VAR1u, that specifies the Var1 protein. The Cox4 targeting signal is fused to the N-terminus to direct import of Var1. Complements var1 mutations in mtDNA.
    Depositor
    Thomas Fox, Thomas Mason
    Article
    Sanchirico et al EMBO J. 1998 Oct 1;17(19):5796-804.
    Insert
    VAR1u, VAR1 recoded for expression from the nucleus
    Use
    Yeast ecoli shuttle vector
    Tags
    preCox4 mitochondrial matrix targeting signal
    Expression
    Yeast
    Mutation
    Synthetic gene in standard genetic code
    Promoter
    ADH1
    Availability
    Academic Institutions and Nonprofits only

  17. HC080

    Plasmid
    #59545
    Purpose
    Expresses yeast Atg13 under the endogenous promoter with a GFP N-terminal tag
    Depositor
    Daniel Klionsky
    Insert
    ATG13 (ATG13 Budding Yeast)
    Use
    Tags
    GFP
    Expression
    Bacterial and Yeast
    Mutation
    AvrII site created after start codon
    Promoter
    ATG13
    Availability
    Academic Institutions and Nonprofits only

  18. pMZ374

    Plasmid
    #59896
    Purpose
    Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: Empty sgRNA target (CspCI placeholder) (see comments).
    Depositor
    Mikel Zaratiegui
    Article
    Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.
    Inserts
    Cas9
    rrk1:sgRNA
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Silent muation of the CspCI site
    Promoter
    adh1 and rrk1
    Availability
    Academic Institutions and Nonprofits only
    59896-plasmid-map-sequence-id-313658

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Showing: 61 - 80 of 6966 results