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Showing: 41 - 60 of 26666 results

  1. pFLIP38

    Plasmid
    #65616
    Purpose
    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.
    Depositor
    Wolf Frommer
    Article
    Jones et al Elife. 2014 Apr 15;3:e01741. doi: 10.755
    Type
    Empty backbone
    Use
    Tags
    6His-edCit-attR1 and attR2-edCer-cMyc
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Availability
    Academic Institutions and Nonprofits only
    65616-plasmid-map-sequence-id-111417

    Enlarge

  2. pFLIP42

    Plasmid
    #65617
    Purpose
    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.
    Depositor
    Wolf Frommer
    Article
    Jones et al Elife. 2014 Apr 15;3:e01741. doi: 10.755
    Type
    Empty backbone
    Use
    Tags
    6His-Cit-attR1 and attR2-mCer-cMyc
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Availability
    Academic Institutions and Nonprofits only
    65617-plasmid-map-sequence-id-111419

    Enlarge

  3. pFLIP43

    Plasmid
    #65618
    Purpose
    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.
    Depositor
    Wolf Frommer
    Article
    Jones et al Elife. 2014 Apr 15;3:e01741. doi: 10.755
    Type
    Empty backbone
    Use
    Tags
    6His-edAFPt9-attR1 and attR2-edCer-cMyc
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Availability
    Academic Institutions and Nonprofits only
    65618-plasmid-map-sequence-id-111420

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  4. pCAPB02

    Plasmid
    #65628
    Purpose
    A transformation-associated recombination (TAR) based genomic capture and expression vector for antibiotic production in B. subtilis
    Depositor
    Pei-yuan Qian
    Article
    Li et al Sci Rep. 2015 Mar 24;5:9383. doi: 10.103
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    65628-plasmid-map-sequence-id-120067

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  16. pET23a_mod_HuD_FL

    Plasmid
    #65751
    Purpose
    plasmid for bacterial overexpression of HuD
    Depositor
    Thomas Tuschl
    Insert
    ELAVL4 (ELAVL4 Human)
    Use
    Tags
    6X His and FLAG HA
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    65751-plasmid-map-sequence-id-114929

    Enlarge

  17. BPK764

    Plasmid
    #65767
    Purpose
    Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Bacterial
    Mutation
    Promoter
    T7 (x2)
    Availability
    Academic Institutions and Nonprofits only
    65767-plasmid-map-sequence-id-112648

    Enlarge

  18. MSP712

    Plasmid
    #65768
    Purpose
    Bacterial expression plasmid for Sp-dCas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSpdCas9(D10A/H840A)-T7-BsaIcassette-Sp-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes dCas9 (D10A/H840A)-NLS-3XFlag, and SpCas9 gRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Bacterial
    Mutation
    D10A and H840A mutations in Cas9
    Promoter
    T7 (x2)
    Availability
    Academic Institutions and Nonprofits only
    65768-plasmid-map-sequence-id-112655

    Enlarge

  19. MSP1673

    Plasmid
    #65769
    Purpose
    Bacterial expression plasmid for S. thermophilus1 Cas9 & sgRNA (need to clone in spacer into BspMI sites): T7-humanSt1Cas9-NLS-T7-BspMIcassette-St1-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS, and St1Cas9 gRNA
    Use
    CRISPR
    Tags
    NLS
    Expression
    Bacterial
    Mutation
    Promoter
    T7 (x2)
    Availability
    Academic Institutions and Nonprofits only
    65769-plasmid-map-sequence-id-112659

    Enlarge

  20. BPK2101

    Plasmid
    #65770
    Purpose
    Bacterial expression plasmid for S. aureus Cas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSaCas9-NLS-3xFLAG-T7-BsaIcassette-Sa-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized SaCas9, and SaCas9 gRNA
    Use
    CRISPR
    Tags
    NLS-3xFlag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 (x2)
    Availability
    Academic Institutions and Nonprofits only
    65770-plasmid-map-sequence-id-112650

    Enlarge

Showing: 41 - 60 of 26666 results