pUCP20 PilBL193*
(Plasmid #162855)

• Purpose: Expression of PilB L193 Amber with a C-terminal histag
• Depositing Lab: Katrina Forest
• Publication: Forest Lab PilB Plasmids (unpublished)

Backbone

• Vector backbone: pUCP20
• Backbone size w/o insert (bp): 3643
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PilB
• Species: Pseudomonas aeruginosa
• Insert Size (bp): 1728
• Mutation: L193Amber
• Tag / Fusion Protein:
  • Histidine tag (H6x) (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• A portion of this plasmid was derived from a plasmid made by: clone from pUCP20 PilB

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Amber mutation was inserted concomitant to replacing the original UAG stop codon with UAA from pUCP20 PilB