pMV2 AAV-RapaInducible-NFZ-HGF
(Plasmid
#188749)
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PurposeRapamycin inducible AAV vector expressing HGF
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Depositing Labs
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 188749 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepJT360
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHGF
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SpeciesH. sapiens (human)
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Entrez GeneHGF (a.k.a. DFNB39, F-TCF, HGFB, HPTA, SF)
- Promoter Rapamycin inducible
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Vector has small deletion in the 5' ITR region; not recommended for AAV production until corrected
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMV2 AAV-RapaInducible-NFZ-HGF was a gift from Michael Bassik & Lacra Bintu (Addgene plasmid # 188749 ; http://n2t.net/addgene:188749 ; RRID:Addgene_188749) -
For your References section:
Development of compact transcriptional effectors using high-throughput measurements in diverse contexts. Tycko J, Van MV, A, DelRosso N, Yao D, Xu X, Ludwig C, Spees K, Liu K, Hess GT, Gu M, Mukund AX, Suzuki PH, Kamber RA, Qi LS, Bintu LB, Bassik MC.. bioRxiv. (PrePrint) 10.1101/2023.05.12.540558
