pADsacA
(Plasmid #158649)

• Purpose: Constitutive transcription of sacA crRNA and GFP
• Depositing Lab: Wenjing Cui
• Publication: Cui Lab Plasmids (unpublished)

Backbone

• Vector backbone: pAD123
• Backbone size w/o insert (bp): 5952
• Vector type: Bacterial Expression, CRISPR, Synthetic Biology ; Shuttle vector Bacillus subtilis / E. coli
• Selectable markers: Chloramphenicol

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Select for growth in Bacillus subtilis using Chloramphenicol.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: sacA crRNA
• Alt name: homologous arm of sacA
• gRNA/shRNA sequence: AATTTCTACTGTTGTAGATTCGGCCGCCTCGATTATAACAAG
• Species: Francisella tularensis subsp. novicida
• Insert Size (bp): 1409
• Promoter: Pveg

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: gggttattgtctcatgagcgg

Resource Information

• Supplemental Documents:
  • pADsacA.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note: Plasmid contains an E295G mutation in rep60. This mutation is not expected to affect plasmid function.