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Addgene

pGAL - FFL[SEL]
(Plasmid #1102)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1102 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pRS316 (pBluescript, Ura3)
  • Backbone size w/o insert (bp) 4400
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    firefly luciferase
  • Species
    firefly
  • Insert Size (bp)
    1800
  • Mutation
    n/a
  • GenBank ID
    M15077

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site Kpn1 (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer M13R
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

induction by shifting to galactose media; peroxisomal targeting signal mutated (enzyme localized in cytoplasm)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGAL - FFL[SEL] was a gift from Susan Lindquist (Addgene plasmid # 1102 ; http://n2t.net/addgene:1102 ; RRID:Addgene_1102)
  • For your References section:

    Multiple effects of trehalose on protein folding in vitro and in vivo. Singer MA, Lindquist S. Mol Cell 1998 Apr;1(5):639-48. 10.1016/S1097-2765(00)80064-7 PubMed 9660948